Vitamin B2, B3 & B5 Test Panel

What

This test panel measures the levels of vitamin B2, vitamin B3, vitamin B5 in your blood.

Who

This test panel is for anyone who suspects they may be deficient in vitamin B2, B3, or B5, or has a condition that makes it difficult to absorb certain nutrients.

How

Blood

Where

Find a location near you

Schedule an appointment, then visit a Quest Diagnostics patient service center location.

About the test

The Vitamin B2, B3 & B5 Test Panel measures the levels of the following 3 essential vitamins in your blood: vitamin B2 (riboflavin), vitamin B3 (niacin), and vitamin B5 (Pantothenic acid).

Vitamins B2, B3, and B5 help convert the food you eat into usable energy and play key roles in nerve function, red blood cell production, hormone synthesis, and metabolism of fats, carbohydrates, and proteins. Low or imbalanced levels may contribute to fatigue, weakness, skin or nerve symptoms, or difficulty maintaining normal energy levels.

Identifying and addressing nutritional deficiencies are important steps toward better health. Your body does not produce most nutrients, so you must get them through food or supplements. How nutrients work in your body is influenced by more than just what you eat. These factors include genetics, age, health conditions, medications, metabolism, and how well your body absorbs nutrients.

This test can help you and your healthcare provider identify whether your B-vitamin levels may be contributing to symptoms such as low energy or whether your diet or supplements are meeting your body’s needs. Results can also be useful for monitoring levels if you follow a restrictive diet, have absorption concerns, or are taking B-vitamin supplements.

No doctor’s visit needed. Buy this vitamin B blood test and visit a nearby Quest location.

This test may be right for you if you follow a specific or restrictive diet, are concerned about not getting certain nutrients, or are experiencing symptoms that suggest a nutrient deficiency.

Consider the Vitamin B2, B3 & B5 Test Panel if you:

Follow a restrictive diet
Do not consume a well-balanced diet or if your diet is low in nutrients (highly processed food)
Have a condition that may inhibit the absorption of the essential B vitamins

This test panel gives you and your healthcare provider detailed information about the levels of vitamin B2, B3, and B5 in your body.

This test panel measures the levels of three nutrients: vitamin B2, vitamin B3, and vitamin B5 in your blood.

The Vitamin B2 Test measures the level of vitamin B2, also known as riboflavin, in your blood. Riboflavin is a water-soluble vitamin used to turn food into energy. It also helps make red blood cells and keeps your nervous system healthy.

If you don't eat enough riboflavin-rich foods, or if your body doesn't store or absorb enough of it, you may have low levels. Too little riboflavin may cause hair loss, anemia, liver problems, cracked lips, rashes, fatigue, blurry vision, personality changes or depression. Early detection is important to prevent irreversible damage over time, especially to the liver and reproductive system, brain, and heart. A long-term deficiency can lead to cataracts and some cancers.

Our bodies don't make riboflavin, so we need to get it daily from food or supplements. Riboflavin is found naturally in foods such as leafy greens, sweet potatoes, sardines, beef liver, chicken breast, salmon, spinach, almonds, eggs, and milk.

The Vitamin B3 Test measures the level of vitamin B3, also known as nicotinamide or nicotinic acid in your blood. Nicotinamide/nicotinic acid functions as a coenzyme in the body, with over 400 enzymes dependent on it for different reactions. Nicotinamide/nicotinic acid is a water-soluble vitamin that helps turn food into energy, build and repair DNA, and protect cells from damage.

If you don't eat enough nicotinamide/nicotinic acid-rich foods, or if your body doesn't store or absorb enough of it, you may have low levels. Too little nicotinamide/nicotinic acid may cause depression, tiredness, headache, memory loss, swollen mouth, a bright red tongue, mouth sores, constipation, diarrhea, or stomach pain. Early detection is important to prevent irreversible damage over time, especially to the liver and reproductive system, brain, and heart.

Our bodies don't make nicotinamide/nicotinic acid, so we need to get it daily from food or supplements. Nicotinamide/nicotinic acid is found naturally in foods such as red meat, chicken, fish, asparagus, sweet potatoes, mushrooms, bananas, brown rice, and legumes.

The Vitamin B5 Test measures the level of vitamin B5, also known as pantothenic acid, in your blood. Pantothenic acid is a water-soluble vitamin that helps the body turn food into energy. It also helps make and break down fatty acids.

If you don't eat enough pantothenic acid-rich foods, or if your body doesn't store or absorb enough of it, you may have low levels. Not having enough pantothenic acid may cause muscle cramps, headaches, nausea, vomiting, stomach cramps, extreme tiredness, irritability, sleep problems, or burning or numbness in the hands or feet.

Our bodies don't make pantothenic acid, so we need to get it daily from food or supplements. Pantothenic acid is found naturally in foods such as meat, brown rice, nuts, milk, mushrooms, avocados, and broccoli.

Please note: It is important you are hydrated before having your samples collected. These tests, as a group of markers, are intended to provide personal vitamin deficiency insights to empower you to achieve an optimal balance with your lifestyle. These tests are not intended to be a replacement for routine medical monitoring. Individuals partaking in these tests are encouraged to seek professional medical assistance regarding the medical and lifestyle applications of any test results.

Purchaser: You must be 18 years of age or older to purchase a test on questhealth.com.

Patient: This test can be performed on patients between 10 and 100 years old. If this test will be performed on someone less than 18 years old:

The appropriate age bracket needs to be selected during purchase
Both parent/guardian (hereinafter "parent") and patient information will need to be provided during test registration
Both parent and patient must be present for sample collection, the parent will need to have their identification available at collection
Results will be provided to the listed parent

Sample collection: This test requires sample collection at a Quest Diagnostics location. Collection is not available in AK, AZ, or HI.

Please note: Laboratory testing for children under 18 years of age should always be conducted with the guidance of a qualified healthcare provider. Our tests are designed to support continuity of care, ease of access, and empower families with accessible health information. They are not a substitute for medical evaluation or ongoing care from a pediatrician. Parents should review test results with their child's healthcare provider to ensure appropriate interpretation, follow-up, and treatment in the context of the rest of your child's care. If your child is experiencing symptoms or you have concerns about their health, please contact your pediatrician or seek other medical care immediately.

Fasting (not eating or drinking anything except water) for 8-12 hours is required. Certain supplements and medications can interfere with your test results. If you are concerned about any of your supplements or medications impacting your results, talk to your healthcare provider before your sample is collected to see if you should stop taking them and for how long.

After you provide your specimen, it generally takes up to 8 business days to process and release your test results. However, processing times may vary. Your test results will be available in a clear and easy-to-read report. If you have or create a MyQuest® account, you may be able to review your new results there with your other Quest results. Independent healthcare providers are available to discuss questions or concerns you may have regarding your results.

If this test is performed on someone less than 18 years old, the results will be provided to the registered parent.

How it works

questhealth.com offers 150+ consumer-initiated Quest Diagnostics lab tests to empower you to have more control over your health journey. Choose from a variety of test types that best suit your needs.

1

Buy a lab test

No doctor visit is required for purchase, but an independent healthcare provider is involved throughout the process.

Shop now

2

Register your test
& schedule an appointment

Book a visit to one of 2000+ Quest locations.

Register your test

3

Visit Quest for sample collection

Complete your test sample collection during your scheduled appointment.

4

Get fast results

Access results online as soon as they’re available. You will be emailed when they are ready. Discuss your results with an independent healthcare provider at no extra cost. Treatment options may be available.

B vitamins are building blocks for our cells. They support brain and heart function, digestion, and hormone production. They also help turn the food we eat into energy.

FAQs

Would taking supplements be helpful if I consume a diet that restricts foods containing B vitamins?

The best way to get essential vitamins and minerals is from food. However, if your body cannot absorb certain nutrients properly, or you are not able to get enough of them through your diet, then you may consider taking a supplement for that nutrient. B vitamins are water-soluble. A water-soluble vitamin dissolves in water and is easily absorbed by the body. Your body does not store these vitamins for long, so any extra will be eliminated in your urine. Because of this, it is important to consume them every day.

While it is unlikely to get too much of a nutrient if you are taking a supplement, getting too much, known as vitamin toxicity, is possible. It is recommended to confirm a deficiency through testing and talk with your doctor prior to taking supplements.

What are enzymes and coenzymes?

An enzyme is a protein that helps to start, or speed up, a reaction in the body. A coenzyme is a non-protein molecule that helps an enzyme to perform that reaction.

Why is this test only available for children 10 years of age or older?

Quest has worked with independent physicians to define appropriate age eligibility ranges for each test. These ranges ensure that our offerings are medically responsible and suitable for self-directed use. We plan to review age eligibility for our tests over time as more data and guidance becomes available.

Can I use my FSA or HSA funds to purchase tests at questhealth.com?

You may be able to use your FSA or HSA funds to purchase lab tests at questhealth.com. Please review your FSA/HSA plan or check with a tax professional to confirm specific requirements for individual eligibility.

Tests purchased on questhealth.com are only for individuals who intend to pay directly for testing and do not want to submit a claim for reimbursement under their health insurance.